Malaysian Journal of Analytical Sciences Vol 20 No 6 (2016): 1365 - 1372

DOI: http://dx.doi.org/10.17576/mjas-2016-2006-15

 

 

 

ENZYMATIC GLYCEROLYSIS OF METHYL LAURATE UTILIZING Candida antarctica Lipase b

 

(Gliserolisis Berenzim oleh Metil Laurat Menggunakan Lipase b Candida antarctica)

 

Norul Naziraa Ahmad Jamlus, Jumat Salimon, Darfizzi Derawi*

 

School of Chemical Sciences and Food Technology, Faculty of Science and Technology,

Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia

 

*Corresponding author: darfizzi@ukm.edu.my

 

 

Received: 1 February 2015; Accepted: 29 August 2016

 

 

Abstract

Enzymatic glycerolysis using Candida antarctica lipase b (CALB) as catalyst was studied. Production of monolaurin was optimized based on two selected types of CALB, varying enzyme concentrations and reaction time. Novozyme-435 (N-435) and Aspergillus oryzae were used in this study. About 47.6% of monolaurin was synthesized using N-435 at a 5% enzyme concentration within 24 hours of reaction time. Characterizations were performed using different techniques namely gas chromatography-flame ionization detector (GC-FID), thin layer chromatography (TLC) and Fourier transform infrared spectroscopy (FTIR) analysis.

 

Keywords:  methyl laurate, enzymatic glycerolysis, monolaurin, Novozyme-435, Aspergillus oryzae

 

Abstrak

Tindak balas gliserolisis berenzim menggunakan lipase b Candida antarctica (CALB) telah dikaji. Penghasilan monolaurin dioptimumkan berasaskan dua jenis CALB, kepekatan enzim dan masa tindak balas. Novozym-435 (N-435) dan Aspergillus oryzae digunakan dalam kajian ini. Sebanyak 47.6% monolaurin telah berjaya disintesis menggunakan N-435 pada kepekatan enzim 5% dalam tempoh 24 jam masa tindak balas. Pencirian dijalankan menggunakan teknik analisis berbeza seperti kromatografi gas (GC), kromatografi lapisan nipis (TLC) dan spektroskopi inframerah transfomasi Fourier (FTIR).

 

Kata kunci:  metil laurat, gliserolisis berenzim, monolaurin, Novozym-435, Aspergillus oryzae

 

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